Real-Time PCR Method for Detection of Pathogenic Yersinia enterocolitica in Food
                    
                        
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                    چکیده
منابع مشابه
Development of a PCR-based method for detection of pathogenic Yersinia enterocolitica in pork
Thisted Lambertz, S. 2005. Development of a PCR-based method for detection of pathogenic Yersinia enterocolitica in pork. Doctor ́s dissertation. ISSN 1652-6880, ISBN 91-576-6922-8 During the last decade, Yersinia enterocolitica has been reported to cause between 500 and 800 cases of human gastroenteritis per year in Sweden. As pigs are the only animals in human food production that regularly ha...
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and Implications The ELISA assay is a more rapid and sensitive method for PCR product detection than conventional gel electrophoresis. The PCR/ELISA allows for the processing of numerous samples, is relatively inexpensive and eliminates the need for electrophoretic and photographic equipment as well as the use of potential carcinogens such as ethidium bromide which is utilized in the gel electr...
متن کاملReal - time TaqMan PCR for Yersinia enterocolitica detection based on 1 the ail and foxA genes
1 Corresponding author. Mailing address: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, 155 Changbai Road Changping District 102206, Beiing, People’s Republic of China. Phone: 8610 58900758. Fax: 8610-58900070. E-mail: [email protected], [email protected] a ...
متن کاملReal-time TaqMan PCR for Yersinia enterocolitica detection based on the ail and foxA genes.
Yersinia enterocolitica, a cause of emerging enteric infections, is a foodborne pathogen associated with various enteric and systemic syndromes, e.g., diarrhea, enteritis, and enterocolitis. Therefore, the detection of this pathogen has important significance. Previous real-time PCR for detection of Y. enterocolitica was primarily based on the ail gene; biotype 1A nonpathogenic strains were not...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00405-08